The value of Alcian blue periodic acid Schiff staining and Ki-67 expression in diagnosing gastric reactive epithelial hyperplasia and dysplasia.
10.3760/cma.j.cn112151-20211125-00860
- VernacularTitle:阿辛蓝过碘酸雪夫染色和Ki-67表达在胃黏膜反应性增生与异型增生鉴别诊断中的价值
- Author:
Zhong Yue SHI
1
;
Wei Hua HOU
2
;
Ying WANG
1
;
Zhong Qiu TIAN
1
;
Qing CAO
1
;
Xin Meng GUO
1
;
Jun LU
1
;
Xue LI
1
;
Hong CHEN
1
;
Mu Lan JIN
1
Author Information
1. Department of Pathology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
2. Department of Pathology, People's Liberation Army Joint Logistics Support Force 989 Hospital (formerly 152 Hospital), Pingdingshan 467099, China.
- Publication Type:Journal Article
- MeSH:
Alcian Blue;
Humans;
Hyperplasia;
Ki-67 Antigen/metabolism*;
Periodic Acid;
Staining and Labeling;
Stomach Neoplasms/diagnosis*
- From:
Chinese Journal of Pathology
2022;51(8):713-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the clinicopathological characteristics of reactive epithelial hyperplasia and dysplasia in the stomach, as well as the clinical value of mucin special staining and proliferating cell nuclear antigen (Ki-67) in distinguishing the two gastric lesions. Methods: The clinical pathological data of 63 patients with gastric reactive epithelial hyperplasia, 54 patients with low-grade dysplasia, and 63 patients with high-grade dysplasia diagnosed from May 2018 to May 2021 in Beijing Chaoyang Hospital, Capital Medical University, Beijing, China were analyzed. Alcian blue periodic acid Schiff (AB-PAS) and Ki-67 staining were performed to examine the mucin staining pattern, number of Ki-67 positive cells, Ki-67 staining patterns in the three groups of lesions, and histopathologic characteristics. Results: The positive rates of AB-PAS in the reactive epithelial hyperplasia and gastric dysplasia groups were 87.3%(55/63) and 10.3%(12/117), respectively. The expression of AB-PAS in the reactive epithelial hyperplasia was gradually increased from the base to the surface of the epithelium. In low-grade dysplasia and high-grade dysplasia, there was no mucin present in the dysplasia epithelium. The difference between the two groups was statistically significant (P<0.01). The positive rate of Ki-67 in the epithelial reactive hyperplasia (>10%) was 81.0% (51/63), and the positive cells were mainly located in the neck and middle parts of the mucosal glands (58/63, 92.1%). In the low-grade dysplasia group, the positive rate of Ki-67 (>10%) was 90.7%(49/54); the positive cells were mainly located in the upper mucosa (33/54, 61.1%), showing a banded distribution pattern; in the high-grade dysplasia group, the positive rate (>10%) was 95.2%(60/63), and the positive cells were mainly located in the whole mucosa (49/63, 77.8%), showing a diffuse/diffuse scattered distribution pattern. The three groups had statistically different rates and distribution patterns of Ki-67 expression (P<0.01). Conclusion: The gastric epithelial reactive hyperplasia and dysplasia can be differentiated using clinicopathological features, AB-PAS staining and Ki-67 expression pattern.
