Detection and clinical application of HIV-1 DNA.
10.3760/cma.j.cn112338-20211230-01036
- Author:
Li Juan DONG
1
;
Hui Chao CHEN
1
;
Yan Ling MA
1
;
Wenge XING
2
Author Information
1. Institute for AIDS/STD Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming 650022, China.
2. National HIV/AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
- Publication Type:Journal Article
- MeSH:
Humans;
HIV-1/genetics*;
HIV Infections/diagnosis*;
DNA;
Polymerase Chain Reaction;
HIV Seropositivity
- From:Chinese Journal of Epidemiology
2022;43(10):1685-1690
- CountryChina
- Language:Chinese
-
Abstract:
The persistence of the HIV-1 reservoir is still the main obstacle to the cure of HIV. In clinical research, reliable biomarkers are needed to label it. HIV-1 DNA can be continuously detected in the HIV-1 reservoir. It has significant application value in diagnosing HIV-1 infection, the timing of antiretroviral therapy, the prediction of virus rebound, and monitoring treatment effects. The detection technology based on polymerase chain reaction (PCR) is the most commonly used HIV-1 DNA detection method in clinical practice. The continuous innovation and advancement of technology can accurately detect the total, integrated, and unintegrated HIV-1 DNA in infected cells using qualitative or quantitative methods. Different forms of HIV-1 DNA in infected cells have been increasingly reported as biomarkers in HIV infection monitoring and AIDS treatment-related research. This article reviews the progress of HIV-1 DNA.
