Molecular characteristics of ciprofloxacin-cefotaxime-azithromycin co-resistant Salmonella enterica Serovar Thompson in foodborne diseases in Hunan Province.
10.3760/cma.j.cn112150-20220112-00046
- Author:
Mi LU
1
;
Wei Shuai ZHAI
1
;
Peng Cheng DU
2
;
Yang WANG
1
;
Zhi Fei ZHAN
3
;
Shuai CHEN
3
;
Hua Yun JIA
3
;
Li BAI
4
Author Information
1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
2. Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100102, China.
3. Microbiological Laboratory, Hunan Provincial Center for Disease Control and Prevention, Changsha 410028, China.
4. Division I of Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Humans;
Ciprofloxacin;
Cefotaxime;
Azithromycin;
Serogroup;
Phylogeny;
Drug Resistance, Multiple, Bacterial/genetics*;
Anti-Bacterial Agents/pharmacology*;
Salmonella;
Quinolones;
Foodborne Diseases;
Plasmids;
Salmonella enterica;
Microbial Sensitivity Tests
- From:
Chinese Journal of Preventive Medicine
2022;56(12):1745-1750
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the molecular characteristics of ciprofloxacin-cefotaxime-azithromycin co-resistant Salmonella enterica serovar Thompson (S. Thompson) isolates from sporadic cases of foodborne diseases and aquatic foods in Hunan province. Methods: Ciprofloxacin-cefotaxime-azithromycin co-resistant S. Thompson isolates were selected from samples, and broth microdilution method was used to determine the resistance to 11 antibiotics of these isolates in vitro. Whole genome sequencing was used for investigating antimicrobial resistance gene patterns and phylogenetic relationships of strains. Results: Nine ciprofloxacin-cefotaxime-azithromycin co-resistant isolates were recovered from 19 S. Thompson isolates. Among nine ciprofloxacin-cefotaxime-azithromycin co-resistant isolates, eight of them harbored IncC plasmids, simultaneously carrying plasmid-mediated quinolone resistance (PMQR) genes qepA and qnrS1, β-lactamase resistance gene blaCMY-2, azithromycin resistance gene mph(A), and one isolate harbored IncR plasmid, and carried PMQR genes qnrB4 and aac(6')-Ib-cr, blaOXA-10 and mph(A). Genetic environment analysis showed that qnrS1, qepA, mph(A) and blaCMY-2 genes might be integrated on genomes of strains by ISKra4, IS91, IS6100 and ISEcp1, respectively. Phylogenetic core genome comparisons demonstrated that ciprofloxacin-cefotaxime-azithromycin co-resistant isolates from patients and aquatic foods were genetically similar and clustered together. Conclusion: Ciprofloxacin-cefotaxime-azithromycin co-resistant S. Thompson isolates have been isolated from both human and aquatic food samples, suggesting that the spread of multidrug resistant Salmonella between human and aquatic animals.
