Cytotoxicity and biocompatibility of Zirconia (Y-TZP) posts with various dental cements.
10.5395/rde.2016.41.3.167
- Author:
Hyeongsoon SHIN
1
;
Hyunjung KO
;
Miri KIM
Author Information
1. Department of Conservative Dentistry, Asan Medical Center, Ulsan University, Seoul, Korea. kmrne@hanmail.net
- Publication Type:Original Article
- Keywords:
Biocompatibility;
Cytotoxicity;
Resin cement;
Zirconia
- MeSH:
Cytokines;
Dental Cements*;
Enzyme-Linked Immunosorbent Assay;
Fibroblasts;
Interleukin-6;
Osteoblasts;
Polymerase Chain Reaction;
Resin Cements;
Reverse Transcription;
RNA, Messenger;
Tooth
- From:Restorative Dentistry & Endodontics
2016;41(3):167-175
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Endodontically treated teeth with insufficient tooth structure are often restored with esthetic restorations. This study evaluated the cytotoxicity and biological effects of yttria partially stabilized zirconia (Y-TZP) blocks in combination with several dental cements. MATERIALS AND METHODS: Pairs of zirconia cylinders with medium alone or cemented with three types of dental cement including RelyX U200 (3M ESPE), FujiCEM 2 (GC), and Panavia F 2.0 (Kuraray) were incubated in medium for 14 days. The cytotoxicity of each supernatant was determined using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays on L929 fibroblasts and MC3T3-E1 osteoblasts. The levels of interleukin-6 (IL-6) mRNA were evaluated by reverse transcription polymerase chain reaction (RT-PCR), and IL-6 protein was evaluated by enzyme-linked immunosorbent assays (ELISA). The data were analyzed using one-way ANOVA and Tukey post-hoc tests. A p < 0.05 was considered statistically significant. RESULTS: The MTT assays showed that MC3T3-E1 osteoblasts were more susceptible to dental cements than L929 fibroblasts. The resin based dental cements increased IL-6 expression in L929 cells, but reduced IL-6 expression in MC3T3-E1 cells. CONCLUSIONS: Zirconia alone or blocks cemented with dental cement showed acceptable biocompatibilities. The results showed resin-modified glass-ionomer based cement less produced inflammatory cytokines than other self-adhesive resin-based cements. Furthermore, osteoblasts were more susceptible than fibroblasts to the biological effects of dental cement.
