Analysis on identification and traceability of one non-toxigenic Corynebacterium diphtheriae from a patient with diabetic foot in Hainan province.
10.3760/cma.j.cn112150-20211111-01045
- VernacularTitle:海南省一例糖尿病患者足趾感染非产毒白喉棒状杆菌的鉴定与溯源分析
- Author:
Xiao Jun ZHOU
1
;
Cun Ren CHEN
2
;
Xu Ming WANG
1
;
Hua WU
1
;
Tao HUANG
1
;
Shao Ling WANG
3
;
Lina NIU
4
Author Information
1. Clinical Laboratory Department, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, China.
2. Endocrinology Department, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, China.
3. Hainan Center for Disease Control & Prevention, Haikou 570203, China.
4. Key Laboratory of Translational Medicine for Tropical Diseases, Hainan Medical University, Haikou 571199, China.
- Publication Type:Journal Article
- MeSH:
Aged;
Corynebacterium/genetics*;
Corynebacterium diphtheriae/genetics*;
Diabetes Mellitus;
Diabetic Foot;
Diphtheria/microbiology*;
Humans;
RNA, Ribosomal, 16S/genetics*
- From:
Chinese Journal of Preventive Medicine
2022;56(8):1107-1111
- CountryChina
- Language:Chinese
-
Abstract:
There is a rare case of an elderly diabetic with diabetic foot infection at Hainan General Hospital in September 2021, which was diagnosed as Corynebacterium diphtheriae infection incidentally on routine culture with conventional methods and molecular biological approaches, to aid in diagnosis in clinical practice. Owing to smear staining, Albert staining and VITEK 2 system, automated identification systems viz matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) confirmed combing with 16S ribosomal RNA (16S rRNA) gene has been used for the taxonomic classification of bacteria. Otherwise, toxin gene tox was done for diphtheria toxin synthesis. The isolate was Gram-stain-positive, rod-like arrangement with irregular thickness, with characteristic metachromatic granules, ferment most sugars and homology of 16S rRNA analyses with C. diphtheriae NCTC11397T (MW682323.1) was greater than a 100% possibility, toxin gene tox was negative. The findings lay the foundation to clinical identify and trace of non-toxigenic C. diphtheriae. Moreover, this work provides insights into the non-toxigenic C.diphtheriae that contribute to recognized risk of non-toxigenic C.diphtheriae infections.
