Loss of Heterozygosity in Oligodendroglioma by Real-time Quantitative Polymerase Chain Reaction-based Microsatellite Analysis
- VernacularTitle:实时荧光定量PCR微卫星分析技术检测少突胶质细胞肿瘤染色体1p、19q和10q杂合性缺失
- Author:
Jiang SU
;
Tao JIANG
;
Zhenrong SUN
;
Guilin LI
;
Fang YUAN
- Publication Type:Journal Article
- Keywords:
oligodendroglioma, loss of heterozygosity, polymerase chain reaction
- From:
Chinese Journal of Rehabilitation Theory and Practice
2011;17(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo set up the new lab examination method for 1p, 19q and 10q loss of heterozygosity(LOH) in glioma.MethodsThirty-eight cases of oligodendroglioma were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on tumor tissues in order to study the status of chromosomes 1p, 19q and 10q.ResultsAmong the 38 cases of oligodendroglioma, 25 cases (65.7%) showed 1p LOH, 26 cases (68.4%) showed 19q LOH, while 5 cases (13.2%) showed 10q LOH.ConclusionReal-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific for detecting LOH in glioma tissues.
