Effect of Dose Compatibility of Main Chemical Components from Astragali Radix- Angelicae Sinensis Radix on Proliferation of VSMCs Based on Uniform Design
10.13422/j.cnki.syfjx.20220451
- VernacularTitle:基于均匀试验设计的黄芪-当归主要化学成分剂量配伍对血管平滑肌细胞增殖的影响
- Author:
Lingbo CHEN
1
;
Ruibin REN
2
;
Huifang YAN
1
;
Changqing DENG
1
Author Information
1. College of Integrated Traditional Chinese and Western Medicine，Medical School， Hunan University of Chinese Medicine，Changsha 410208，China
2. School of Mathematics，Southwest Jiaotong University，Chengdu 611756，China
- Publication Type:Journal Article
- Keywords:
uniform design;
regression analysis;
quadratic polynomial;
Astragali Radix;
Angelicae Sinensis Radix;
chemical composition;
half inhibitory concentrationn （IC50）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(3):143-151
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe functional model of six major components of Astragali Radix-Angelicae Sinensis Radix combination against the proliferation of vascular smooth muscle cells （VSMCs） was constructed by uniform design， the relationship between the compatibility of these six main components and the inhibition of VSMCs proliferation was analyzed， and the effect of the compatibility of these main components of Astragali Radix-Angelicae Sinensis Radix on the proliferation of VSMCs as well as the feasibility of uniform design test in the study of multi-component compatibility of Chinese medicines were discussed. MethodCell proliferation and toxicity assay kit （CCK-8） method was used to determine the inhibitory effect of the six components of Astragali Radix-Angelicae Sinensis Radix on platelet derived growth factor-BB （PDGF-BB）-induced VSMCs proliferation in rats and the half inhibitory concentration （IC50） of each component were obtained. Six chemical components of Astragali Radix-Angelicae Sinensis Radix （formononetin， astragaloside Ⅰ， astragaloside Ⅳ， calycosin， ferulic acid and calycosin-7-O-β-D-glucoside） were taken as the independent variables X1， X2， X3， X4， X5， X6， respectively， and the cell proliferation inhibition rate as the dependent variable Y. U24*
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（249） uniform design table and mathematical models （including linear and quadratic polynomial） were selected， the stepwise regression method was used to screen the variables， and the better equations were obtained in theory. The actually better relationship equations were screened by setting the different dose compatibility of these six components for verification tests. According to the purpose of the experiment， the selected regression equation was processed by data centralization， so as to compare the regression coefficients of the data centralization equation and analyze the correlation between components and efficacy. ResultThe six components of Astragali Radix-Angelicae Sinensis Radix could inhibit abnormal proliferation of VSMCs in a dose-dependent manner. IC50 values of formononetin， astragaloside Ⅰ， astragaloside Ⅳ， calycosin， ferulic acid and calycosin-7-O-β-D-glucoside were 123.453， 113.184， 81.655， 141.159， 101.187， 151.016 mg·L-1， respectively. Two superior models were selected by experimental verification， including equation 1 of Y1=73.137 897-0.207 888X1+0.000 508X22+0.000 347X1X6 and equation 2 of Y2=70.038 433-0.236 665X1+0.000 577X22+0.000 260X1X4+0.000 415X1X6+0.000 370X3X5. After data centralization， equation 2 was adjusted to Y=-1.480 260-0.146 281X1+0.000 673X22+0.000 314X1X4+0.000 456X1X6+0.000 737X3X5， the regression coefficients showed that the astragaloside Ⅰ had a positive effect on the inhibition rate of cell proliferation， formononetin-calycosin， formononetin-calycosin-7-O-β-D-glucoside and astragaloside Ⅳ-ferulic acid had an interaction effect on the inhibition rate of cell proliferation， and the synergistic effect of astragaloside Ⅳ and ferulic acid was higher than other interaction items. ConclusionThe combination of six main components of Astragali Radix-Angelicae Sinensis Radix can inhibit the abnormal proliferation of VSMCs， and it is demonstrated that the uniform test design can be used to predict the correlation between the chemical composition of Chinese medicines with their pharmacological effects， which can provide an experimental basis for the rational use of uniform design and regression analysis to construct mathematical models to study the compatibility relationship of Chinese medicines.
