Anti-convulsant Activity of Goat Horn and Its Effect on Neurotransmitters in Mouse Brain
10.13422/j.cnki.syfjx.20221613
- VernacularTitle:山羊角抗惊厥活性评价及其对小鼠脑内神经递质的影响
- Author:
Zhaoying ZHU
1
;
Wenxing WU
1
;
Jingjing ZHAO
1
;
Rui LIU
1
;
Sheng GUO
1
;
Jin'ao DUAN
1
Author Information
1. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine，Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，and National Administration of Traditional Chinese Medicine Key Laboratory for Chinese Medicine Resources Recycling Utilization，Nanjing University of Chinese Medicine， Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
goat horn;
electroshock-induced convulsion model;
neurotransmitter;
ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(3):133-142
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the anti-convulsant activity of goat horn and its effect on the main neurotransmitters in the brain of model mice. MethodA total of 120 mice were randomly divided into a blank group，a model group，a positive drug group （estazolam， 1 mg·kg-1）， a Saigae Tataricae Cornu （STC， 0.2 g·kg-1） group， and low- （1.25 g·kg-1） and high-dose （2.5 g·kg-1） goat horn groups. Drugs were administered by gavage once at 10 mL·kg-1. Those in the blank group and the model group were given the same volume of 0.2% sodium carboxymethyl cellulose （CMC-Na）. The mice underwent threshold electrical stimulation once 2 h after administration. Ankylosing convulsion in the hind limbs of mice was used as the index to calculate the recovery period， convulsion rate， and mortality of ankylosing convulsion. Ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） was established to analyze the changes in the content of the main neurotransmitters in the brain tissues of mice. ResultTwo hours after intragastric administration of goat horn powder to mice， the recovery period of convulsion could be shortened and the convulsion rate and mortality were reduced （P<0.05）. In terms of anti-convulsant effect， the groups were ranked as follows： high-dose goat horn group > STC group > low-dose goat horn group. UPLC-MS/MS analysis showed that compared with the results in the model group， goat horn powder and STC decreased the content of glutamic acid and aspartic acid （P<0.05） and increased the content of glycine， γ-aminobutyric acid， and 5-hydroxytryptamine （P<0.05）. ConclusionThe results showed that goat horn had a significant anti-convulsant effect on the electroshock-induced convulsion mouse model， and the underlying mechanism was presumably attributed to the improvement of the development of convulsion by regulating the changes in neurotransmitter content in brain tissues.
