Effect of Jianpi Xiaoai Prescription on Expression of Metastasis-related Factors in Tumor Microenvironment of Nude Mouse Model of Liver Metastasis of Colon Cancer
10.13422/j.cnki.syfjx.20230128
- VernacularTitle:健脾消癌方对结肠癌肝转移裸鼠模型肿瘤微环境转移相关因子表达的影响
- Author:
Weihua HE
1
;
Lan DENG
2
;
Yilan JIANG
3
Author Information
1. Graduate School， Hunan University of Chinese Medicine， Changsha 410208， China
2. Affiliated Hospital of Jiangxi University of Chinese Medicine， Nanchang 330006， China
3. Affiliated Hospital of Hunan Academy of Chinese Medicine， Changsha 410006， China
- Publication Type:Journal Article
- Keywords:
Jianpi Xiaoai prescription;
colorectal cancer;
liver;
metastasis;
tumor microenvironment
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(3):81-87
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of Jianpi Xiaoai prescription on the protein expression of metastasis-related factors in tumor microenvironment such as chemokine receptor 4 （CXCR4）， transformation growth factor-β （TGF-β）， integrin av β5 （ITGαv β5）， serum calcium-binding protein A4 （S100A4）， serum calcium-binding protein A8 （S100A8）， and serum calcium-binding protein A9 （S100A9） in the liver tissue of the nude mouse model of liver metastasis of colon cancer， and to explore the possible mechanism of its anti-liver metastasis of colon cancer. MethodBALB/c nude mice were randomly divided into blank group， model group， and Jianpi Xiaoai prescription low， medium， and high-dose groups. A nude mouse model of liver metastasis of human colon cancer was established. Jianpi Xiaoai prescription low， medium， and high-dose groups were given 5.4， 10.8， 21.6 g·kg-1 liquid medicine， respectively， and the model group and the blank group were given the same volume of distilled water by gavage， once a day for 3 consecutive weeks. 24 h after the last administration， the nude mice were sacrificed by neck removal， and the liver metastasis of each group was observed. Western blot was used to determine the protein expression of metastasis-related factors in the tumor microenvironment， such as CXCR4， TGF-β， ITGαv β5， S100A4， S100A8， and S100A9. ResultThe proportion of metastatic tumor area was 73% in the model group， 72% in the low-dose group， 55% in the medium-dose group， and 42% in the high-dose group. The high-dose group was significantly lower than the model group （P<0.05）. As compared with the model group， the expression of CXCR4， TFG-β， ITGαv β5， S100A8， and S100A9 in the Jianpi Xiaoai prescription high and medium-dose groups were significantly decreased （P<0.05）. As compared with the model group， the expression of S100A4 in the Jianpi Xiaoai prescription high， medium， and low-dose groups was significantly decreased （P<0.05）. The expression of CXCR4， TFG-β， ITGαv β5， S100A8， and S100A9 in the Jianpi Xiaoai prescription low-dose group was not significantly different from that in the model group. ConclusionJianpi Xiaoai prescription can inhibit liver metastasis of colon cancer， and its mechanism may be related to the reduction of the expression of metastasis-related factors such as CXCR4， TGF-β， ITGαv β5， S100A4， S100A8， and S100A9 in tumor microenvironment.
