Shoutaiwan Ameliorates Oxidative Damage of Human Chorionic Trophoblast Cells by Regulating Nrf2 Signaling Pathway to Treat Recurrent Abortion
10.13422/j.cnki.syfjx.20222240
- VernacularTitle:寿胎丸通过调控Nrf2信号通路减轻人绒毛膜滋养层细胞的氧化损伤治疗复发性流产
- Author:
Sinan SHEN
1
;
Zhenni MU
1
;
Li TANG
1
;
Lei LEI
1
Author Information
1. College of Integrated Chinese and Western Medicine， Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases， Hunan University of Chinese Medicine， Changsha 410208， China
- Publication Type:Journal Article
- Keywords:
Shoutaiwan;
oxidative stress;
apoptosis;
human chorionic trophoblast cells;
nuclear factor erythroid 2-related factor 2
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(3):44-51
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the protective effect of Shoutaiwan-containing serum on the human chorionic trophoblast cells （HTR-8/Svneo） exposed to hydrogen peroxide （H2O2） via the nuclear factor erythroid 2-related factor 2 （Nrf2） signaling pathway and to decipher the underlying mechanism. MethodThe H2O2 solutions of 25， 50， 100， 200， 400 μmol·L-1 were used to treated the HTR-8/Svneo cells. The cell counting kit-8 （CCK-8） was employed to measure the proliferation of the cells and further determine the optimal concentration of H2O2 solution for modeling and the drug-containing serum. The cells were divided into a blank group， a model group， a dydrogesterone group， and a Shoutaiwan group. The effect of drug-containing serum on H2O2-induced proliferation of HTR-8/Svneo cells was detected by CCK-8 assay. The intracellular reactive oxygen species （ROS） in each group was determined by enzyme-linked immunosorbent assay （ELISA）. Western blot was employed to determine the protein levels of Nrf2， heme oxygenase-1 （HO-1）， quinone oxidoreductase 1 （NQO1）， cysteine-containing aspartate-specific protease-3 （Caspase-3）， and B cell lymphoma/leukemia-2 （Bcl-2）. Cellular immunofluorescence was employed to detect the expression of Nrf2 and Bcl-2. Real-time quantitative polymerase chain reaction （Real-time PCR） was carried out to examine the mRNA level of Nrf2， Caspase-3， and Bcl-2 associated X protein （Bax）. ResultThe optimal concentration of H2O2 for modeling was 50 μmol·L-1， and the optimal concentration of drug-containing serum was 10%. Compared with the blank group， the modeling decreased the proliferation of cells （P<0.01）， increased the intracellular ROS （P<0.01）， down-regulated the protein levels of Nrf2， HO-1， NQO1， and Bcl-2 （P<0.05， P<0.01）， up-regulated the protein levels of Caspase-3 and Bax （P<0.01）， down-regulated the mRNA level of Nrf2 （P<0.01）， and up-regulated the mRNA levels of Caspase-3 and Bax （P<0.05， P<0.01）. Compared with the model group， Shoutaiwan-containing serum increased the proliferation of cells （P<0.01）， reduced the intracellular ROS （P<0.01）， up-regulated the protein levels of Nrf2， HO-1， NQO1， and Bcl-2 （P<0.01）， down-regulated the protein levels of Caspase-3 and Bax （P<0.05， P<0.01）， up-regulated the mRNA level of Nrf2 （P<0.05）， and down-regulated the mRNA levels of Caspase-3 and Bax （P<0.05， P<0.01）. ConclusionShoutaiwan-containing serum can inhibit H2O2-induced apoptosis by activating the Nrf2 signaling pathway and has protective effect on human chorionic trophoblast cells.
