Correlation analysis of ocular demodex infection and the composition of meibum lipid flora
10.3980/j.issn.1672-5123.2023.1.25
- VernacularTitle:眼部蠕形螨感染影响睑酯菌群组成的相关分析
- Author:
Pei-Yan ZHU
1
;
Shao-Qin LIN
1
;
Wan-Ying LIN
1
;
Mu-Ling LI
1
;
Hong-Ying FAN
1
;
Qiong-Xi LIN
1
;
Yu-Han FENG
1
;
Jing XU
1
;
Juan YANG
1
;
Qiong LIU
1
Author Information
1. Department of Ophthalmology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
- Publication Type:Journal Article
- Keywords:
demodex;
meibum lipid flora;
meibomian gland dysfunction;
dry eye;
correlation analysis
- From:
International Eye Science
2023;23(1):126-131
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the correlation between the ocular demodex infection and the composition of meibum lipid flora.METHODS: A non-interventional and observational study was performed on recruited 39 patients in our hospital between July 2020 and February 2021. They were divided into control group(n=14), meibomian gland dysfunction(MGD)group(n=14), and demodex group(FM, n=11)according to the presence or absence of demodex infection or MGD. High-throughput sequencing of V3-V4 fragment of 16S rRNA gene was performed on the meibomian ester samples of the three groups of subjects, and bioinformatics analysis was performed on the sequencing data to study the composition and difference of meibum lipid flora in the subjects of ocular demodex.RESULTS: Pseudomonas and Comamonas in FM group were significantly higher than those in control group and MGD group(P<0.05), while Ralstonia in Demodex infection group was significantly lower than that in control group and MGD group(P<0.05). The microbial richness and community diversity of meibum lipid flora of the MGD group and the FM group were significantly higher than those of the control group(P<0.05).CONCLUSION: Ocular demodex infection changed the composition of meibum lipid flora and increased the microbial richness and community diversity of meibum lipid flora.
