Mechanism of Electroacupuncture on Preventing Apoptosis of Hippocampal Neurons in Rats with Acute Sleep Deprivation and Its Effect on Anxiety
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0611
- VernacularTitle:四神聪电针预防急性睡眠剥夺大鼠海马神经元凋亡的机制及对焦虑行为的影响
- Author:
Bo-yu JIAO
1
;
Fan-qi MENG
1
;
Xing-ru XIAO
2
;
Jing-yun ZHANG
2
;
Wen-ya PEI
1
;
Jing-wen RUAN
1
Author Information
1. Department of Acupuncture and Moxibustion， The First Affiliated hospital Sun Yat-sen University，Guangzhou 510080，China
2. Zhongshan School of Medicine，Sun Yat-sen University，Guangzhou 510080，China
- Publication Type:Journal Article
- Keywords:
sleep deprivation;
electroacupuncture;
neuronal apoptosis;
astrocytes;
open field test
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(6):958-966
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism of Sishencong electroacupuncture on preventing the apoptosis of hippocampal neurons in rats with acute sleep deprivation and its effect on anxiety behavior. MethodsAdult Sprague-Dawley （SD） rats were randomly divided into Sham group （control group）， SD group （sleep deprivation group）， and EA+SD group （eletroacupuncture group）. Modified multiple platform method was used to induce sleep deprivation for 4 days. EA+SD group received Sishencong electroacupuncture in each day during sleep deprivation with 20 minutes needling for 4 days. The open field test was used to detect the exploration of rats to evaluate the degree of anxiety， and the co-labeling of NeuN+ and Cleaved Caspase-3 in different regions of the hippocampus was detected by immunofluorescence staining； the astrocyte marker glial fibrillary acidic protein （GFAP） in the hippocampus was labeled by immunofluorescence. GFAP and the morphological changes of astrocytes were analyzed by Sholl analysis， and the co-labeling with C3+ and S100A+ were detected to analyze the A1 and A2 types of astrocytes in the hippocampus. ResultsOpen field test showed that the time spent in the center of rats was increased in EA+SD group， but the movement speed was decreased compared with SD group. Immunofluorescence staining confirmed that the expression of Cleaved caspase-3 on neurons in different regions of the hippocampus （CA1， CA2， CA3， DG1， DG2） in the EA+SD group was lower than that of the SD group （P<0.05）. The number of intersections of astrocytes and concentric circles in the EA+SD group at 12 μm，15 μm， 18 μm and 21 μm were higher than those of the SD group. Immunofluorescence showed that electroacupuncture could regulate the proportion of C3+/GFAP+ and S100A+/GFAP+ in the hippocampus of rats during sleep deprivation. ConclusionElectroacupuncture could improve the exploration during sleep deprivation by reducing the apoptosis of neurons in the hippocampus， repairing the morphological damage of astrocytes and balancing the proportion of A1 and A2 astrocytes in the hippocampus.
