Preliminary study on the effect of Pinggan huoxue powder on cerebral ischemia-reperfusion injury in rats and its mechanism
- VernacularTitle:平肝活血散对大鼠脑缺血再灌注损伤的影响及作用机制初探
- Author:
Faping SONG
1
;
Jie ZHANG
1
;
Yong TANG
2
;
Zongmei LIU
1
;
Xiaoling WANG
2
Author Information
1. The First Clinical College，Shaanxi University of Chinese Medicine，Shaanxi Xianyang 712000，China
2. Dept. of Neurology，Ankang Hospital of Traditional Chinese Medicine，Shaanxi Ankang 725000，China
- Publication Type:Journal Article
- Keywords:
Pinggan huoxue powder;
cerebral ischemia-
- From:
China Pharmacy
2022;33(24):2999-3004
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect and mechanism of Pinggan huoxue powder on cerebral ischemia-reperfusion injury （CIRI） in rats. METHODS CIRI model was induced by suture-occluded method. SD rats were divided into sham operation group， model group， positive control group （Tongxinluo capsule 0.325 g/kg）， Pinggan huoxue powder low-dose， medium-dose and high-dose groups （3.02， 6.04， 12.08 g/kg）， with 12 rats in each group. Each group was given constant volume of water or drug intragastrically， once a day， for consecutive 14 d. Longa score was used to evaluate neurological function of rats on the 1st， 7th and 14th day of intervention. After the last administration， the percentage of cerebral infarction volume in rats was measured， and neuronal injury （neuronal density） and neuronal apoptosis were observed； the protein expressions of p53， B cell lymphoma-2 （Bcl-2）， Bcl-2 associated X protein （Bax）， Caspase-3 and activated Caspase-3 （Cleaved caspase-3） in brain tissue were detected. RESULTS Compared with sham operation group， Longa score at the 1st， 7th and 14th day of intervention and the percentage of cerebral infarction volume in model group significantly increased （P＜0.05）， and the arrangement of cortical cells in ischemic side was disordered， Nissl bodies were absent and vacuolated， and a large number of cell apoptosis occurred. The neuronal density and protein expression of Bcl-2 at the lesion side were significantly decreased （P＜0.05）， while the protein expressions of p53， Bax， Caspase-3 and Cleaved Caspase-3 were significantly increased （P＜0.05 or P＜0.01）. Compared with model group， the expressions of Bcl-2 in cerebral ischemia cortex of rats in Pinggan huoxue powder high-dose group were significantly increased （P＜0.05）. Longa scores of rats in Pinggan huoxue powder medium-dose and high-dose groups on the 7th and 14th day of intervention were significantly decreased （P＜0.05）， and other indexes were also significantly reversed （P＜0.05 or P＜0.01）. CONCLUSIONS Pinggan huoxue powder can protect cerebral nerves and inhibit apoptosis of CIRI model rats， the mechanism of which may be related to down-regulating the protein expressions of p53， Bax and Caspase-3 and up-regulating the protein expression of Bcl-2.
