Investigation of the improvement effect of Dihuang yinzi on neurological function of APP/PS1 double transgenic positive mice based on gut-brain axis and its mechanism
- VernacularTitle:基于肠脑轴探讨地黄饮子对APP/PS1转基因阳性小鼠神经功能的改善作用及机制
- Author:
Jing CHEN
1
;
Xicai LIANG
2
;
Jian WANG
2
;
Shenghua WANG
2
;
Tianyi NING
2
Author Information
1. Teaching Experiment Center of Liaoning University of Traditional Chinese Medicine，Shenyang 110847，China
2. Experimental Animal Center of Liaoning University of Traditional Chinese Medicine，Shenyang 110847，China
- Publication Type:Journal Article
- Keywords:
Dihuang yinzi;
gut-brain axis;
neurological
- From:
China Pharmacy
2022;33(24):2978-2984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the improvement effect of Dihuang yinzi on neurological function of APP/PS1 double transgenic positive mice and its possible mechanism. METHODS APP/PS1 double transgenic positive mice were randomly divided into APP/PS1 positive model group， Dihuang yinzi low-dose， medium-dose and high-dose groups （12， 24， 48 g/kg，by the amount of crude drug）， western medicine group （donepezil hydrochloride 0.8 mg/kg）； APP/PS1 transgenic negative mice were included in as APP/PS1 negative normal group， with 6 mice in each group. Administration groups were given relevant medicine intragastrically； APP/PS1 positive model group and APP/PS1 negative normal group were given constant volume of normal saline intragastrically， once a day， for consecutive 28 d. After the last medication， the ability of learning and memory， intestinal flora diversity （only medium-dose group of Dihuang yinzi）， pathological changes of neural cells in hippocampus， and the expressions of Bcl-2， Bax and brain-derived neurotrophic factor （BDNF） in mice of each group were investigated. RESULTS Compared with APP/PS1 positive model group， escape latency of mice was shortened significantly in Dihuang yinzi medium-dose and high-dose groups， while the times of crossing the platform significantly increased （P＜0.05）. Compared with APP/PS1 positive model group， Chao1 index of Dihuang yinzi medium-dose group was lower， while Shannon index was higher （P＜0.05）； OTU abundance of microbial flora was decreased to some extent； dominant flora was Clostridia o_ Clostridia_ vadinBB60_ Group， verruca G_ UCG_ 005. Compared with the APP/PS1 positive model group， the pathological changes of the nerve cells in the hippocampal CA1 area of mice were improved in the medium-dose and high-dose groups of Dihuang yinzi； the protein expressions of Bcl-2 and BDNF in the hippocampal tissue were significantly increased， while the protein expression of Bax was significantly decreased （P＜0.05）. CONCLUSIONS Dihuang yinzi can enhance the diversity of flora， change the type of dominant flora， and can inhibit the apoptosis of brain neurons， and increase the level of BDNF； its mechanism may be to protect nerve cells by means of gut-brain axis， thereby improving the learning and memory ability of APP/PS1 positive model mice.
