HSV-1 H129-Derived Anterograde Neural Circuit Tracers: Improvements, Production, and Applications

Hong Yang; Feng Xiong; Yi-ge Song; Hai-fei Jiang; Hai-bin Qin; Jing Zhou; Wen-bo Zeng; Min-hua Luo; Hong Yang; Feng Xiong; Yi-ge Song; Hai-fei Jiang; Hai-bin Qin; Jing Zhou; Min-hua Luo; Sha LU; Steven F Grieco; Xiangmin XU; Fei Zhao; Fei Zhao; Min-hua Luo

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HSV-1 H129-Derived Anterograde Neural Circuit Tracers: Improvements, Production, and Applications

Author: Hong YANG ¹ ; Feng XIONG ¹ ; Yi-Ge SONG ¹ ; Hai-Fei JIANG ¹ ; Hai-Bin QIN ¹ ; Jing ZHOU ¹ ; Wen-Bo ZENG ¹ ; Min-Hua LUO ¹ ; Hong YANG ² ; Feng XIONG ² ; Yi-Ge SONG ² ; Hai-Fei JIANG ² ; Hai-Bin QIN ² ; Jing ZHOU ² ; Min-Hua LUO ² ; Sha LU ³ ; Steven F. GRIECO ⁴ ; Xiangmin XU ⁴ ; Fei ZHAO ⁵ ; Fei ZHAO ⁶ ; Min-Hua LUO ⁷
Author Information

1. State Key Laboratory of Virology, CAS Center for Excellence in Brain Science and Intelligence Technology, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences
2. University of Chinese Academy of Sciences
3. Shanghai Genechem Co. Ltd.
4. Department of Anatomy and Neurobiology, School of Medicine, University of California
5. School of Basic Medical Sciences, Capital Medical University
6. Chinese Institute for Brain Research
7. Shanghai Public Health Clinical Center, Fudan University
Publication Type:Journal Article
Keywords: Anterograde transneuronal tracer; Application; HSV-1 strain H129 (H129); Monosynaptic tracer; Neural circuit; Neural circuit tracing; Polysynaptic tracer; Production
From: Neuroscience Bulletin 2021;37(5):701-719
CountryChina
Language:Chinese
Abstract: Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. They have been developed from herpes simplex virus 1 (HSV-1) strain H129 (H129), and have been successfully applied to map diverse neural circuits. Initially, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT, which was highly successful, yet improvements are needed. Now, by inserting another tdTomato expression cassette into the H129-dTK-tdT genome, we have created H129-dTK-T2, an updated version of H129-dTK-tdT that has improved labeling intensity. To help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.