Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography

Liyuan Jiang; Chengjun LI; Miao LI; Tianding WU; Chunyue Duan; Yong Cao; Jianzhong HU; Liyuan Jiang; Chengjun LI; Miao LI; Tianding WU; Chunyue Duan; Yong Cao; Jianzhong HU; Liyuan Jiang; Hongbin LU; Jianzhong HU; Chengjun LI; Miao LI; Tianding WU; Chunyue Duan; Yong Cao; Hongbin LU; Liyuan Jiang; Chengjun LI; Miao LI; Tianding WU; Chunyue Duan; Yong Cao; Hongbin LU; Jianzhong HU; Liyuan Jiang; Chengjun LI; Miao LI; Tianding WU; Chunyue Duan; Yong Cao; Hongbin LU; Jianzhong HU; Xianzhen Yin

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Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography

Author: Liyuan JIANG ¹ ; Chengjun LI ¹ ; Miao LI ¹ ; Tianding WU ¹ ; Chunyue DUAN ¹ ; Yong CAO ¹ ; Jianzhong HU ¹ ; Liyuan JIANG ² ; Chengjun LI ² ; Miao LI ² ; Tianding WU ² ; Chunyue DUAN ² ; Yong CAO ² ; Jianzhong HU ² ; Liyuan JIANG ³ ; Hongbin LU ³ ; Jianzhong HU ³ ; Chengjun LI ⁴ ; Miao LI ⁴ ; Tianding WU ⁴ ; Chunyue DUAN ⁴ ; Yong CAO ⁴ ; Hongbin LU ⁴ ; Liyuan JIANG ⁵ ; Chengjun LI ⁵ ; Miao LI ⁵ ; Tianding WU ⁵ ; Chunyue DUAN ⁵ ; Yong CAO ⁵ ; Hongbin LU ⁵ ; Jianzhong HU ⁵ ; Liyuan JIANG ⁶ ; Chengjun LI ⁶ ; Miao LI ⁶ ; Tianding WU ⁶ ; Chunyue DUAN ⁶ ; Yong CAO ⁶ ; Hongbin LU ⁶ ; Jianzhong HU ⁶ ; Xianzhen YIN ⁷
Author Information

1. Department of Spine Surgery, Xiangya Hospital, Central South University
2. National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University
3. Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province
4. Department of Sports Medicine, Xiangya Hospital, Central South University
5. Xiangya Hospital-International Chinese Musculoskeletal Research Society Sports Medicine Research Centre
6. Hunan Engineering Research Center of Sport and Health
7. Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Publication Type:Journal Article
Keywords: 3D; High-resolution; Neurovascular; Spinal cord; Srμct
From: Neuroscience Bulletin 2021;37(10):1469-1480
CountryChina
Language:Chinese
Abstract: Effective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases. However, ideal techniques for simultaneously imaging neurovascular structure in a broad region of a specimen are still lacking. In this study, we combined Golgi staining with angiography and synchrotron radiation micro-computed tomography (SRμCT) to visualize the 3D neurovascular network in the mouse spinal cord. Using our method, the 3D neurons, nerve fibers, and vasculature in a broad region could be visualized in the same image at cellular resolution without destructive sectioning. Besides, we found that the 3D morphology of neurons, nerve fiber tracts, and vasculature visualized by SRμCT were highly consistent with that visualized using the histological method. Moreover, the 3D neurovascular structure could be quantitatively evaluated by the combined methodology. The method shown here will be useful in fundamental neuroscience studies.