Down-regulated expression of NPM1 in IMS-M2 cell line by (-)-epigallocatechin-3-gallate
10.12980/APJTB.4.2014APJTB-2014-0177
- Author:
Hoang Thanh CHI
1
;
Phu Chi DUNG
1
;
Phan Thi XINH
1
;
Bui Thi Kim LY
2
;
Hoang Anh VU
3
;
Phan Thi XINH
3
;
Yuko SATO
4
Author Information
1. Department of Molecular Cytogenetics, Hematology and Blood Transfusion Hospital in Ho Chi Minh City
2. Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
3. Center for Molecular Biomedicine, The University of Medicine and Pharmacy-Ho Chi Minh City
4. Basic nursing science, The Japanese Red Cross College of Nursing Japan
- Publication Type:Journal Article
- Keywords:
Apoptosis;
EGCG;
IMS-M2;
NPM1
- From:Asian Pacific Journal of Tropical Biomedicine
2014;4(7):570-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Methods: Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis. Results: EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells. Conclusions: The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.
