Antimicrobial activity against periodontopathogenic bacteria, antioxidant and cytotoxic effects of various extracts from endemic Thermopsis turcica
10.12980/APJTB.4.2014APJTB-2013-0010
- Author:
Elif Burcu BALI
1
;
Leyla AÇIK
2
;
Mecit VURAL
2
;
Gülçin AKCA
3
;
Meral SARPER
4
;
Mualla Pinar ELÇI
4
;
Ferit AVCU
5
Author Information
1. Health Services Vocational School, University of Gazi
2. Department of Biology, Faculty of Science, University of Gazi
3. Department of Medical Microbiology, Faculty of Dentistry, University of Gazi
4. Cancer and Stem Cell Research Center, Gulhane Military Medical School
5. Department of Hematology, Cancer and Stem Cell Research Center, Gulhane Military Medical School
- Publication Type:Journal Article
- Keywords:
Acute promyelocytic leukemia;
Antimicrobial activity;
Antioxidant effect;
Cytotoxic effect;
Human gingival fibroblast;
Periodontopathogenic bacteria;
Phenolic content;
Thermopsis turcica
- From:Asian Pacific Journal of Tropical Biomedicine
2014;4(7):505-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan, Vural&Küçüködük against periodontopathogenic bacteria, its antioxidant activity and cytotoxic effect on various cancer cell lines. Methods: In vitro antimicrobial activities of ethanol, methanol, ethyl acetate (EtAc), n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion, minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antioxidant properties of the extracts were evaluated by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and β-carotene bleaching methods. Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent. Additionally, cytotoxic activity of the extracts on androgen-insensitive prostate cancer, androgen-sensitive prostate cancer, chronic myelogenous leukemia and acute promyelocytic leukemia human cancer cell lines were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Human gingival fibroblast cells were used as a control. Results: Our data showed that EtAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemcomitans (MIC: 1.562 mg/mL, MBC: 3.124 mg/mL) and Porphyromonas gingivalis (MIC: 0.781 mg/mL, MBC: 1.562 mg/mL). In antioxidant assays, EtAc extract exhibited also the highest radical scavenging activity [IC
