In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.)
10.12980/APJTB.4.2014APJTB-2014-0462
- Author:
Wanigasekera Daya RATNASOORIYA
1
;
Walimuni Prabhashini Kaushalya Mendis ABEYSEKERA
2
;
Chatura Tissa Dayendra RATNASOORIYA
3
Author Information
1. Faculty of Allied Health Sciences, General Sir John Kothalawala Defence University
2. Herbal Technology Section, Industrial Technology Institute
3. Faculty of Medicine, University of Colombo
- Publication Type:Journal Article
- Keywords:
Anti-aging;
Anti-hyaluronidase;
Black tea;
Camellia sinensis;
Hyaluronic acid;
Orange pekoe;
Sri Lankan tea
- From:Asian Pacific Journal of Tropical Biomedicine
2014;4(12):959-963
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification. Epigallocatechin gallate (EGCG) was used as the reference agent (concentrations tested: 0.012, 0.025, 0.050, 0.100 and 0.200 mg/mL). Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid (from rooster comb) and bovine testicular hyaluronidase. Results: The results revealed that BTB had moderate [IC