Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes
10.12980/APJTB.4.201414B364
- Author:
Emmanuel Anyachukwu IRONDI
1
;
Ganiyu OBOH
1
;
Afolabi Akintunde AKINDAHUNSI
1
;
Emmanuel Anyachukwu IRONDI
2
;
Aline Augusti BOLIGON
3
;
Margareth Linde ATHAYDE
3
Author Information
1. Department of Biochemistry, Federal University of Technology
2. Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University
3. Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria
- Publication Type:Journal Article
- Keywords:
Diabetic complications;
Enzyme inhibition;
Hyperglycemia;
Lipid peroxidation;
Mangifera indica;
Mucuna urens
- From:Asian Pacific Journal of Tropical Biomedicine
2014;4(11):903-910
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ{variant}-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe
