Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: An in vitro study
10.12980/APJTB.4.2014APJTB-2014-0059
- Author:
Hakan SÖYÜT
1
;
Elif Duygu KAYA
2
;
Şükrü BEYDEMIR
3
Author Information
1. Department of Primary Education, Faculty of Education, Bayburt University
2. Department of Food Engineering, Faculty of Engineering, Igdir University
3. Department of Chemistry, Faculty of Science, Atatürk University
- Publication Type:Journal Article
- Keywords:
Cefoperazone sodium;
Inhibition;
Meropenem trihydrate;
Paraoxonase;
Piperacillin sodium
- From:Asian Pacific Journal of Tropical Biomedicine
2014;4(8):603-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). Methods: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. Results: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. Conclusions: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.
