miR-218 expression in osteosarcoma tissues and its effect on cell growth in osteosarcoma cells
- Author:
Hong-Tai WANG
1
;
Ai-Gang LIU
1
;
Zhang-Nan ZHOU
1
;
Hong-Guang LIN
1
;
Rong-Zi CHEN
1
;
Kun CHEN
1
;
Dao-Shu LUO
2
;
Jin-Shui HE
3
Author Information
- Publication Type:Journal Article
- Keywords: Apoptosis; MiR-218; Osteosarcoma; Proliferation
- From: Asian Pacific Journal of Tropical Medicine 2014;7(12):1000-1004
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the expression of miR-218 and its clinical significance in osteosarcoma tissues and explore its effect on proliferation and apoptosis in osteosarcoma cells. Methods: miR-218 expression was detected in 76 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR). MiR-218 was over-expressed by exogenous miR-218 plasmids in Saos-2 cells, and then BrdU cell proliferation assay and flow cytometry were used to determine cell proliferation and apoptosis. Results: The expression of miR-218 in osteosarcoma tissues was significantly lower than those in normal tumor-adjacent tissues (t=8.735, P<0.001). MiR-218 expression in tumor tissues was significantly correlated with tumor size (χ
