Evaluation of protective effect of IL-22 and IL-12 on cutaneous leishmaniasis in BALB/c mice
10.1016/S1995-7645(14)60166-8
- Author:
Hajar Ziaei HEZARJARIBI
1
;
Fatemeh GHAFFARIFAR
2
;
Abdolhossein DALIMI
2
;
Zohreh SHARIFI
3
Author Information
1. Department of Parasitology and Mycology, Faculty of Medicine, Mazandaran University of Medical Sciences
2. Parasitology and Entomology Department, Faculty of Medical Sciences, Tarbiat Modares University
3. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine
- Publication Type:Journal Article
- Keywords:
IL-12;
IL-22;
Leishmania major;
Mice;
Protection
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(12):940-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effect of IL-22 and IL-12 on cutaneous leishmaniasisin BALB/c mice. Methods: The protective effect of IL-22 and IL-12 on cutaneous leishmanias in BALB/c mice was evaluated by measurement of IL-4, INF-γ, total IgG, IgG1 and IgG2a after challenge with Leishamania major. Clinical evaluations were performed by measurement of lesion diameter, and survival rate of the mice. Results: In week 27 post infection, the mortality rates for control groups were 100%. While the survival rates for the IL-12, IL-12 + IL-22, and IL-22(5 ng/g) groups were 100%. The size of lesions decreased in the presence IL-22 (5 ng/g) of mice weight, which was statistically significant in comparison with other groups (. P<0.05). Mean of total IgG, IgG1 and IgG2a for IL-22 (5 ng/g) group was more than other groups. In IL-22 group (5 ng/g), INF-γ production was significantly higher than other groups and IL-4 was significantly lower than other groups. Conclusions: The results obtained indicate the effectiveness of IL-22 and its effect on IL-12 in protection of cutaneous leishmaniasis.
