Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development
10.1016/S1995-7645(14)60165-6
- Collective Name:Centre for Biotechnology, Muthayammal College of Arts and Science;Department of Marine Biotechnology, Bharathidasan University;Department of Biotechnology, Vivekanandha College of Engineering for Women;Department of Zoology, Arignar Anna Government Arts College
- Publication Type:Journal Article
- Keywords:
Attenuated live vaccine;
Mutants;
Salmonella enterica serovar Typhi
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(12):933-939
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop attenuated strains of Salmonella enterica serovar Typhi (. S. typhi) for the candidate vaccine by osmolar stress. Methods: S. typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Namakkal, Tamil Nadu, India. Both strains were grown in LB (Luria Bertani) medium supplemented with various concentration of NaCl (0.1-0.7M) respectively. The effect of osmolar stress was determined at molecular level by PCR using MGR 06 and MGR 07 primers corresponding to ompR with chromosomal DNA of S. typhi SS3 and SS5 strains. Attenuation by osmolar stress results in deletion mutation of the S. typhi strains was determined by agglutination assays, precipitation method, SDS PAGE analysis and by animal models. Results: The 799 bp amplified ompR gene product from wild type S. typhi SS3 and SS5 illustrate the presence of virulent gene. Interestingly, there was only a 282 bp amplified product from S. typhi SS3 and SS5 grown in the presence of 0.5, 0.6 and 0.7 M NaCl. This illustrates the occurrence of deletion mutation in ompR gene at high concentration of NaCl. Furthermore, both the wild-type and mutant S. typhi outer membrane SDS-PAGE profile reveals the differences in the expression of ompF, ompC and ompA proteins. In mice, wild type and mutant strains lethal dose (LD
