Monitoring of prostate cancer growth and metastasis using a PSA luciferase report plasmid in a mouse model
10.1016/S1995-7645(14)60153-X
- Author:
Qi-Qi MAO
1
;
Yi-Wei LIN
1
;
Hong CHEN
1
;
Kai YANG
1
;
De-Bo KONG
1
;
Hai JIANG
1
Author Information
1. Department of Urology, First Affiliated Hospital of Zhejiang University Medical College
- Publication Type:Journal Article
- Keywords:
Luciferase report gene;
Metastasis;
Prostate cancer;
Tumor growth
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(11):879-883
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct a PSA luciferase report plasmid and monitor the growth and metastasis of prostate cancer after emasculation in SCID mice. Methods: PSA promoter sequence and luciferase gene were amplified by PCR and subsequently inserted into pZsGreen1-1 vector to construct pPSA-FL-Luc vector. LNCaP cells that were stably transfected with pPSA-FL-Luc were used to establish a SCID mouse xenograft model. Then, the growth and metastasis of prostate cancer were monitored via living imaging. Results: We successfully constructed a PSA luciferase plasmid, pPSA-FL-Luc. DHT enhanced luciferase activity in a concentration-dependent manner in 293T cells with pPSA-FL-Luc transfection. Prostate cancer SCID mouse model was established with pPSA-FL-Luc transfected LNCaP cells. In tumor bearing mice with or without emasculation, pPSA-FL-Luc plasmid was applied to monitored tumor growth and metastasis based on bioluminescence imaging. Conclusions: We construct a pPSA-FL-Luc plasmid, which stably expresses luciferase and can be applied to monitor tumor development in a prostate SCID mouse model.
