In vitro antifilarial activity of Azadirachta indica aqueous extract through reactive oxygen species enhancement
10.1016/S1995-7645(14)60147-4
- Author:
Niladri MUKHERJEE
1
;
Prasanta SAINI
1
;
Suprabhat MUKHERJEE
1
;
Priya ROY
1
;
Santi P. SINHA BABU
1
Author Information
1. Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University
- Publication Type:Journal Article
- Keywords:
Antifilarial;
Azadirachta indica;
Reactive oxygen species;
Setaria cervi
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(11):841-848
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate an aqueous preparation from the Azadirachta indica leaves (AEA) against Setaria cervi (. S. cervi), a model filarial parasite. Method: In vitro efficacy of AEA was evaluated against S. cervi through estimation of relative motility value, dye exclusion test and MTT assay. Visible morphological alterations were monitored using conventional microscopic techniques in microfilariae and haematoxylin-eosin stained sections of AEA-treated adults. Results: Enhancement of reactive oxygen species in S. cervi treated with AEA was established through alteration in the activity of glutathione S-transferase, superoxide dismutase, catalase, peroxidase and level of superoxide anion and reduced glutathione. Conclusion: In vitro filaricidal activity of AEA is possibly through disturbing redox homeostasis by down-regulating and altering the level of some key antioxidants and regulatory enzymes like reduced glutathione, glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxidase of S. cervi.
