In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam.
10.1016/S1995-7645(14)60242-X
- Author:
Manila YADAV
1
;
Amita YADAV
1
;
Jaya Parkash YADAV
1
Author Information
1. Department of Genetics, M.D. University
- Publication Type:Journal Article
- Keywords:
Antioxidant;
DPPH;
Endophytic fungi;
Eugenia jambolana;
H;
TPC
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(S1):S256-S261
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To elucidate the antioxidant activity and total phenolic content (TPC) of ethyl acetate extracts of endophytic fungi isolated from Eugenia jambolana by three different antioxidant assays. Methods: Twenty one different endophytic fungal extracts were screened for presence of various phytochemicals, TPC and in vitro antioxidant activity. TPC was tested by Folin-Ciocalteau reagent based assay. DPPH free radical scavenging, hydrogen peroxide scavenging and reducing power assays were used to evaluate the antioxidant activity. Results: Alkaloids, phenols, flavonoids, saponins, and terpenes were the main phytochemicals presents in all 21 endophytes. A significant positive correlation was found between antioxidant activity and TPC in fungal extracts. There is 36% endophytic extracts having high phenolic content exhibited potent antioxidant activity. Chaetomium sp., Aspergillus sp., Aspergillus peyronelii and Aspergillus niger strain showed the highest antioxidant activity ranging from 50% to 80% having 58 mg/g to 60 mg/g GAE total phenolics. Ascorbic acid used as a standard showed 90% reducing potential. Conclusions: The results reveal that metabolites produced by endophytic fungi isolated from Eugenia jambolana can be a potential source of novel natural antioxidant compounds.
