A variant of DC-SIGN gene promoter associated with resistance to HIV-1 in serodiscordant couples in Burkina Faso
10.1016/S1995-7645(14)60211-X
- Author:
Thérèse Samdapawindé KAGONÉ
1
;
Cyrille BISSEYE
1
;
Albert Théophane YONLI
1
;
Tegwindé Rebeca COMPAORÉ
1
;
Jean Baptiste NIKIÉMA
1
;
Jacques SIMPORE
1
;
Thérèse Samdapawindé KAGONÉ
2
;
Nicolas MÉDA
2
;
Jean TESTA
2
;
Dramane KANIA
2
;
Cyrille BISSEYE
3
;
Virginio PIETRA
4
;
Comlan DE SOUZA
5
Author Information
1. Biomolecular Research Center Pietro Annigoni CERBA, Labiogene University of Ouagadougou
2. Centre MURAZ Bobo-Dioulasso
3. Laboratory of Molecular and Cellular Biology, University of Science and Technique of Masuku (USTM)
4. Health Centre Saint Camille of Nanoro
5. Faculty of Sciences, University of Lomé
- Publication Type:Journal Article
- Keywords:
Burkina Faso;
Genetic resistance;
HIV-1
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(S1):S93-S96
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR5δ32). Methods: Two DC-SIGN and two IRF1 single nucleotide polymorphisms (SNPs) as well as HLA-B57*01 and CCR5δ 32 alleles were genotyped in 51 serodiscordant couples in Burkina Faso. DC-SIGN, IRF1 and HLA-B57*01 genotyping was carried out by real time PCR using TaqMan assays (Applied Biosystems, USA and Sacace Biotechnologies, Italy). CCR5δ 32 deletion was investigated by PCR. Results: The two SNPs of DC-SIGN promoter showed a significant genotypic difference in serodiscordant couples. After multivariate analysis, only the association between DC-SIGN rs2287886 and HIV-1 remained significant (P<0.01). No association was found between IRF1 SNPs and HIV-1 infection. CCR5δ 32 wild type allele was found in 100% of serodiscordant couples. A high frequency of HLA-B57*01 allele was found in the HIV-positive (78%) compared with HIV-negative group (51%), however this difference was no longer significant after the correction of the sex confounding effect in the logistic regression model. Conclusions: Our study suggests a protective role of a variation of DC-SIGN promoter and genetic resistance to HIV-1 in serodiscordant couples in Burkina Faso.
