Investigation of caspase-1 activity and interleukin-1β production in murine macrophage cell lines infected with Leishmania major
10.1016/S1995-7645(14)60205-4
- Author:
Mohammad Reza Mahmoudian SANI
1
;
Mehrdad Moosazadeh MOGHADDAM
2
;
Gholamreza FARNOOSH
2
;
Hossein AGHAMOLLAEI
3
;
Kazem HASSANPOUR
4
;
Ramezan Ali TAHERI
5
Author Information
1. Department of Molecular Medicine and Genetic, Hamedan University of Medical Sciences
2. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences
3. Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University
4. Medical School, Sabzevar University of Medical Sciences
5. Nanobiotechnology Research Centre, Baqiyatallah University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Caspase-1 activity;
Interleukin-1β;
Leishmania major;
Macrophage
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(S1):S70-S73
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the caspase-1 dependent inflammatory pathway activity and interleukin-1β (IL-1β) secretion in murine macrophage cell lines J774G8 infected with Leishmania major (L. major) using caspase-1 activity assay and ELISA. Methods: Novy-MacNeal-Nicolle biphasic medium was applied to produce promastigote form of L. major. Metacyclic promastigotes in the stationary phase were applied to infect macrophage. Caspase-1 activity and IL-1β secretion were assessed by the CPP32/caspase-1 fluorometric protease assay and ELISA IL-1β kits, respectively, with time intervals of 6, 18 and 30 h. Results: Our study showed an increase in caspase-1 activity and IL-1β secretion in infected samples compared to non-infected macrophages. The highest increase in IL-1β production was observed after 6 h of infection. Conclusions: These results arise that the activation of inflammasome pathway could be one of the innate immunity pathways against L. major.
