Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour
10.1016/S1995-7645(14)60254-6
- Author:
Nguyen Thi Mai PHUONG
1
;
Trinh Tat CUONG
2
;
Dang Ngoc QUANG
3
Author Information
1. Institute of Biotechnology, Vietnam Academy of Science and Technology
2. Key Laboratory for Enzyme and Protein Technology, Hanoi University of Science
3. Faculty of Chemistry, Hanoi National University of Education
- Publication Type:Journal Article
- Keywords:
Inflammation;
Macrophage;
Stemona tuberosa;
Toll-like 4 receptor
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(S1):S327-S331
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the anti-inflammatory activity of methyl ferulate (MF) isolated from the roots of Stemona tuberosa (S. tuberosa) Lour (Stemonaceae) in lipopolysaccharide activated macrophage cells. Methods: Methanol extracts of a root powder of S. tuberosa were prepared for isolation of a potential anti-inflammatory agent using ultrasound extraction combined with repeated chromatography on silica gel. After the quantitative analyses, anti-inflammatory activity of the isolated compound was evaluated by measurement of cytokine release, NO generation, expression of cyclooxygenase-2 and phosphorylation of mitogen activated protein kinases including p38 and c-Jun NH2-terminal kinase using quantitative kits and Western blotting with specific antibodies. Results: The isolation process yielded a potential anti-inflammatory compound with a purity level of 99% determined by high performance liquid chromatography. The compound was identified as MF by using nuclear magnetic resonance. MF strongly inhibited the release of pro-inflammatory cytokines from macrophages, including IL-6, TNFα, IFNγ, yet it did not affect the anti-inflammatory cytokine IL-10. Phosphorylation of p38 and c-Jun NH2-terminal kinase were clearly reduced in MF-treated macrophages stimulated with lipopolysaccharide. cyclooxygenase-2 expression and NO generation by macrophages were also suppressed when the cells were treated with MF. Conclusions: The data suggested that MF is a possible inhibitor of the mitogen activated phosphor kinase pathway and could be a potential anti-inflammatory agent isolated for the first time in medicinal plant S. tuberosa.
