Comparative assessment of extraction methods and quantitative estimation of luteolin in the leaves of Vitex negundo Linn. by HPLC
10.1016/S1995-7645(14)60248-0
- Author:
Lubna ABIDIN
1
;
Mohd MUJEEB
1
;
Showkat Rasool MIR
1
;
Shah Alam KHAN
2
;
Aftab AHMAD
3
Author Information
1. Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy
2. Department of Pharmacy, Oman Medical College
3. Health Information Technology Department, Jeddah Community College, King Abdulaziz University
- Publication Type:Journal Article
- Keywords:
Extraction techniques;
HPLC;
Luteolin;
Vitex negundo
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(S1):S289-S293
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To find out the ideal organic solvent and extraction technique for the isolation of luteolin from the leaves of Vitex negundo Linn. (V. negundo) by quantitative estimation of luteolin through high performance liquid chromatography (HPLC) method. Methods: The leaves of V. negundo were identified by a botanist, cleaned, dried under shade and powdered. Maceration, reflux, Soxhlet and ultrasound assisted extraction techniques were used for the extraction of luteolin from the leaves by using four different solvents of varying polarity such as methanol, ethanol, chloroform, and dichloromethane. A simple HPLC method was used to determine the quantity of luteolin in each sample extract. Results: The calibration plot of standard luteolin showed a linear relationship in the concentration range of 100-500 μg/mL with a correlation coefficient, r
