Phospholipase C from Pseudomonas aeruginosa and Bacillus cereus; characterization of catalytic activity
10.1016/S1995-7645(14)60150-4
- Author:
Nooran Sherif ELLEBOUDY
1
;
Mohammad Mabrouk ABOULWAFA
1
;
Nadia Abdel-Haleem HASSOUNA
1
Author Information
1. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University
- Publication Type:Journal Article
- Keywords:
Bacillus cereus;
Catalytic activity;
Phospholipase C;
Pseudomonas aeruginosa;
Substrate specificity
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(11):860-866
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study characteristics of phospholipases C (PLCs), their importance for producing microorganisms as well as the potential of their use for industrial purposes. Method: PLC from Bacillus cereus (B. cereus) D101 was selected as an example of Gram-positive PLCs and PLC from Pseudomonas aeruginosa (P. aeruginosa) D183 of Gram-negative ones. Enzymes were partially purified by ammonium sulfate precipitation followed by membrane dialysis. Partially purified preparations were used to study effect of different factors on activities as well as in substrate specificity tests which were conducted using a turbidimetric assay method. Results: Maximum activity was at pH 7 and 8 and 40 °C for P. aeruginosa PLC, and pH 8-10 and 37 °C for B. cereus PLC. Both PLCs were inhibited by Pi at 5 mM or higher, whereas, PLC from B. cereus only was inhibited by EDTA. Activity of P. aeruginosa PLC was not affected by removing Zn
