Mechanism of PEDF promoting the proliferation of lens epithelial cells in human eyes
10.1016/j.apjtm.2015.10.009
- Author:
Wen-Lei YANG
1
;
Lin ZHANG
1
Author Information
1. Department of Ophthalmology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
- Publication Type:Journal Article
- Keywords:
Epithelial cells;
Eye protein;
Human;
Lens;
Pigment epithelium-derived factor;
Vascular endothelial growth factor
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(11):971-975
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the regulation effect of pigment epithelium-derived factor (PEDF) on the growth of human lens endothelial cells (LECs) and related mechanisms in vivo and in vitro. Methods: In the part of in vivo study, 82 eyes of 82 patients with age-related cataract were included to collect the central lens anterior capsule (diameter at 5.0-5.5 mm) with the informed consent of surgery for patients. The selected specimens were divided into the LECs low density group and high density group with 20 specimens for each group based on hematoxylin and eosin staining results. The relative expression level of PEDF mRNA in LECs was detected by reverse transcription PCR. In the part of in vitro study, LEC line (HLE-B3) was cultured and 50 ng/mL PEDF was added in media for 72 h in PEDF culture group, while normally cultured cells were used as the control group. The percentage of LECs at G
