Roots extracts of Adenophora triphylla var. japonica improve obesity in 3T3-L1 adipocytes and high-fat diet-induced obese mice
10.1016/j.apjtm.2015.10.011
- Author:
Dong-Ryung LEE
1
;
Bong-Keun CHOI
1
;
Young-Sil LEE
2
;
Bong-Keun CHOI
2
;
Seung Hwan YANG
2
;
Joo-Won SUH
2
;
Hae Jin LEE
3
;
Sung-Bum PARK
3
;
Seung Hwan YANG
3
;
Joo-Won SUH
3
;
Tack-Man KIM
4
;
Han Jin OH
5
Author Information
1. NutraPham Tech
2. Center for Nutraceutical and Pharmaceutical Materials, Myongji University
3. Interdisciplinary Program of Biomodulation, Myongji University
4. DONG IL Pharmtec
5. Department of Family Medicine, VIEVIS NAMUH Hospital
- Publication Type:Journal Article
- Keywords:
Adipocytes;
Anti-obesity;
High-fat diet-induced obese mice;
Lipogenesis;
Roots of Adenophora triphylla var. japonica extract
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(11):898-906
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the anti-obesity activity and the action mechanism of the roots of Adenophora triphylla var. japonica extract (ATE) in high-fat diet (HFD)-induced obese mice and 3T3-L1 adipocytes. Methods: The roots of Adenophora triphylla were extracted with 70% ethanol. To demonstrate the compounds, linoleic acid was analyzed by using gas chromatography; and the anti-obesity effects and possible mechanisms of ATE were examined in 3T3-L1 adipocytes and HFD-induced obese mice. Results: Treatment with ATE inhibited the lipid accumulation without cytotoxicity in 3T3-L1 adipocytes. Furthermore, 200 and 400 mg/kg ATE treatment significantly decreased the body weight gain, white adipose tissues (WATs) weight and plasma triglyceride level, while 100 and 200 mg/kg ATE treatment increased the plasma high-density lipoprotein cholesterol level in the HFD-induced obese mice, as compared with the HFD group. Treatment with 200 and 400 mg/kg ATE also lowered the size of adipocytes in adipose tissue and reduced the lipid accumulation in liver. ATE treatment showed significantly lower expression level of adipogenesis-related proteins, such as peroxisome proliferator-activated receptor γ, fatty acid binding protein (aP2), fatty acid synthase in 3T3-L1 adipocytes; and furthermore, decreased peroxisome proliferator-activated receptor γ, aP2, fatty acid synthase, sterol regulatory element binding protein-1c, and lipoprotein lipase mRNA expression levels in WAT of the HFD-induced obese mice. Conclusions: These results suggested that the ATE has an anti-obesity effect, which may be elicited by regulating the expression of adipogenesis and lipogenesis-related genes and proteins in adipocytes and WAT of the HFD-induced obese mice.
