Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells

Samrat BARAL; Youn-Chul KIM; Jungwon SEO; Samrat BARAL; Ramesh PARIYAR; Chi-Su YOON; Dong-Cheol KIM; Sung Yeon KIM; Hyuncheol OH; Youn-Chul KIM; Jungwon SEO; Samrat BARAL; Hyuncheol OH; Youn-Chul KIM; Jungwon SEO; Samrat BARAL; Ramesh PARIYAR; Hyuncheol OH; Youn-Chul KIM; Jungwon SEO; Chi-Su YOON; Dong-Cheol KIM; Hyuncheol OH; Youn-Chul KIM; Jong-Min YUN; Seok O JANG

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Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells

Author: Samrat BARAL ¹ ; Youn-Chul KIM ¹ ; Jungwon SEO ¹ ; Samrat BARAL ² ; Ramesh PARIYAR ² ; Chi-Su YOON ² ; Dong-Cheol KIM ² ; Sung Yeon KIM ² ; Hyuncheol OH ² ; Youn-Chul KIM ² ; Jungwon SEO ² ; Samrat BARAL ³ ; Hyuncheol OH ³ ; Youn-Chul KIM ³ ; Jungwon SEO ³ ; Samrat BARAL ⁴ ; Ramesh PARIYAR ⁴ ; Hyuncheol OH ⁴ ; Youn-Chul KIM ⁴ ; Jungwon SEO ⁴ ; Chi-Su YOON ⁵ ; Dong-Cheol KIM ⁵ ; Hyuncheol OH ⁵ ; Youn-Chul KIM ⁵ ; Jong-Min YUN ⁶ ; Seok O. JANG ⁶
Author Information

1. BK21 Plus Team
2. Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University
3. Graduate School of Oriental Medicine, Wonkwang University
4. Hanbang Body-Fluid Research Center, Wonkwang University
5. Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University
6. Department of Internal Medicine, College of Korean Medicine, Wonkwang University
Publication Type:Journal Article
Keywords: Dendrite; Gastrodiae rhizoma; MAP2; Neural stem cells; Neuronal differentiation; Proliferation
From: Asian Pacific Journal of Tropical Medicine 2015;8(10):792-797
CountryChina
Language:Chinese
Abstract: Objective: To investigate the effects of Gastrodiae rhizoma, a dried root of Gastrodia elata Blume, on proliferation and differentiation of human NSCs derived from embryonic stem cells. Methods: A 70% ethanol extract of Gastrodiae rhizoma (EEGR) was estimated with 4-hydroxybenzyl alcohol as a representative constituent by HPLC. Results: MTT assay showed that the treatment with EEGR increased the viability of NSCs in growth media. Compared to control, EEGR increased the number of dendrites and denritic spines extended from a differentiated NSC. Whereas EEGR decreased the mRNA expression of Nestin, it increased that of Tuj1 and MAP2 in NSCs grown in differentiation media. Immunocytochemical analysis using confocal microscopy also revealed the increased expression of MAP2 in dendrites of EEGR-treated NSCs. Furthermore, EEGR decreased mRNA expression of Sox2 in NSCs grown even in growth media. Conclusions: In conclusion, our study demonstrates for the first time that EEGR induced proliferation and neuronal differentiation of NSCs, suggesting its potential benefits on NSC-based therapies and neuroregeneration in various neurodegenerative diseases and brain injuries.