Correlation between TAMs and proliferation and invasion of type I endometrial carcinoma
10.1016/j.apjtm.2015.07.009
- Author:
Hong-Lin HU
1
;
Han-Song BAI
1
;
Hai-Xia PAN
1
;
Han-Song BAI
2
Author Information
1. Department of Oncology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital
2. Graduate School, Zunyi Medical College
- Publication Type:Journal Article
- Keywords:
Invasion;
Proliferation;
Tumor-associated macrophages;
Type I endometrial carcinoma
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(8):643-650
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the correlation between tumor-associated macrophages and the proliferation and invasion of type I endometrial carcinoma. Methods: Immunohistochemistry was used to investigate the infiltration of macrophages in normal and different types of hyperplastic endometrial lesions. The proliferation and invasion ability of type I endometrial carcinoma cell line RL95-2 influenced by mononuclear macrophage cell line THP-1 (constructed M2 type macrophages) was detected by CCK8 and transwell technologies respectively. Transwell was used to evaluate the recruiting ability of RL95-2 on THP-1 cells. Otherwise, the western blot was also used to detect the expression of CyclinD1 and MMP-2 in RL95-2 with the influence of THP-1. Results: Immunohistochemistry result showed a positive correlation between the number of infiltrating macrophages and the progression of endometrial hyperplasia. THP-1 recruited by RL95-2 could promote its proliferation and invasion and enhance the expression of the CyclinD1 and MMP-2 protein in a time dependent manner (P < 0.05). Conclusions: Increase of the number of infiltrating macrophages and its contribution to the tumor inflammatory microenvironment may result in the development of the type I endometrial carcinoma.
