Correlation of survivin, p53 and Ki-67 in laryngeal cancer Hep-2 cell proliferation and invasion
10.1016/j.apjtm.2015.07.007
- Author:
Shi-Geng PEI
1
;
Xue-Ling WANG
1
;
Qing-Jun ZHANG
1
;
Hong ZHANG
1
;
Ju-Xiang WANG
2
Author Information
1. Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hebei Engineering University
2. Hebei Engineering University Medical College
- Publication Type:Journal Article
- Keywords:
Hep-2;
Ki67;
Laryngeal cancer;
P53;
Survivin;
Transwell
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(8):636-642
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of survivin, p53 and Ki-67 on Hep-2 human laryngeal cancer endothelial cell proliferation and invasion. Methods: Laryngeal squamous cell carcinoma and paracancerous normal tissues were collected, total RNA was extracted from tissues, survivin, p53 and Ki-67 gene mRNA expression levels in laryngeal cancer and the adjacent tissues were detected by Real-time PCR. Human laryngeal cancer Hep-2 epithelial cells were selected, survivin gene was overexpressed, and cell proliferation was detected by MTT. p53 and Ki-67 gene expression changes in overexpressed survivin gene were detected by Western blot. Changes in Hep-2 cell invasive ability were studied when survivin was overexpressed as detected by Transwell invasion assay. Results: In the adjacent tissues, survivin, p53 and Ki-67 gene relative expression levels were 1.72 ± 0.9, 13.7 ± 5.7 and 5.7 ± 1.3, respectively; while in cancer tissues, gene relative expression levels were 53.7 ± 8.3, 66.7 ± 5.2 and 61.0 ± 3.1, respectively, which was significantly increased. As detected by MTT, relative cell survival rate within 12 h of survivin overexpression were: load control group (88.5 ± 1.6)%; overexpressed group (90.3 ± 1.9)%. Transwell invasion assay results indicated that overexpressed survivin could significantly increase the relative survival rate of cells. Conclusions: Expressions of p53, Ki67 and survivin are increased in cancer; and there is a positive correlation between survivin, p53 and Ki67 expressions in laryngeal carcinoma.
