Analysis of human B cell response to recombinant Leishmania LPG3

Mostafa Haji Fatahaliha; Maryam Hosseini; Sanaz Rasolzadeh; Mehdi Yousefi; Mostafa Haji Fatahaliha; Maryam Hosseini; Sanaz Rasolzadeh; Dariush Shane Bandi; Behzad Baradaran; Mehdi Yousefi; Mostafa Haji Fatahaliha; Maryam Hosseini; Sanaz Rasolzadeh; Dariush Shane Bandi; Behzad Baradaran; Mehdi Yousefi; Farhad Jadidi-niaragh

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Analysis of human B cell response to recombinant Leishmania LPG3

Author: Mostafa Haji FATAHALIHA ¹ ; Maryam HOSSEINI ¹ ; Sanaz RASOLZADEH ¹ ; Mehdi YOUSEFI ¹ ; Mostafa Haji FATAHALIHA ² ; Maryam HOSSEINI ² ; Sanaz RASOLZADEH ² ; Dariush Shane BANDI ² ; Behzad BARADARAN ² ; Mehdi YOUSEFI ² ; Mostafa Haji FATAHALIHA ³ ; Maryam HOSSEINI ³ ; Sanaz RASOLZADEH ³ ; Dariush Shane BANDI ³ ; Behzad BARADARAN ³ ; Mehdi YOUSEFI ³ ; Farhad JADIDI-NIARAGH ⁴
Author Information

1. Drug Applied Research Center, Tabriz University of Medical Sciences
2. Immunology Research Center, Tabriz University of Medical Sciences
3. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences
4. Department of Immunology, School of Public Health, Tehran University of Medical Sciences
Publication Type:Journal Article
Keywords: B cell; CD69; Cytokine; Leishmania; LPG3; Vaccine
From: Asian Pacific Journal of Tropical Medicine 2015;8(8):624-629
CountryChina
Language:Chinese
Abstract: Objective: To evaluate the capability of recombinant Leishmania LPG3 and its fragments in the activation of B cells. Methods: In the present study, human B cells were purified from peripheral blood of 10 adult healthy subjects using magnetic-activated cell sorting technique. Subsequently, purified B cells were treated with recombinant LPG3, and its N-terminal and C-terminal fragments at different concentrations (2, 10 and 20 μg/mL). B cell activation was assessed through expression of CD69 molecule by flow cytometry and secretion of IL-6, TNF-α and IL-10 cytokines via enzyme-linked immunosorbent assay following treatment with recombinant antigens. Results: Our results showed that while the recombinant LPG-3 could significantly increase the production of IL-6 and TNF-α (P < 0.05) in B cells, it had no effect on the secretion of IL-10 by B cells. Conclusions: Our study indicated that recombinant LPG-3 and especially its N-terminal fragment could stimulate B cell response as an important immune response component against leishmaniasis. Thus, it seems that it can be considered as an effective adjuvant in vaccine developments against leishmaniasis.