Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L. MILL.) against alloxan-induced diabetes in mice
10.1016/j.apjtm.2015.06.013
- Author:
Ali BERRAAOUAN
1
;
Ziyyat ABDERRAHIM
1
;
Mekhfi HASSANE
1
;
Legssyer ABDELKHALEQ
1
;
Aziz MOHAMMED
1
;
Bnouham MOHAMED
1
Author Information
1. Laboratory of Physiology and Ethnopharmacology-URAC40, Mohammed First University, Faculty of Sciences
- Publication Type:Journal Article
- Keywords:
Alloxan prevention;
Antioxidant effect;
Cactus pear seed oil;
Diabetes mellitus;
Opuntia ficus-indica;
Oxidative stress
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(7):532-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the in vitro antioxidant power of cactus pear seed oil [. Opuntia ficus-indica L. MILL. (CPSO)] and its protective effect against chemically induced diabetes mellitus in mice. Methods: The in vitro antioxidant effect of CPSO was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The preventive effect was conducted on Swiss albino mice treated with CPSO (2 mL/kg, per os), before and after a single intraperitoneal alloxan administration (100 mg/kg). Survival rate, body weight and fasting blood glucose were measured and histopathological analysis of pancreas was performed to evaluate alloxan-induced tissue injuries. Results: CPSO exhibited an antioxidant effect in DPPH scavenging assay. Moreover, the administration of CPSO (2 mL/kg) significantly attenuated alloxan-induced death and hyperglycemia (P < 0.001) in treated mice. Morphometric study of pancreas revealed that CPSO significantly protected islets of langerhans against alloxan induced-tissue alterations. Conclusions: Based on theses results, CPSO can prevente alloxan-induced-diabetes by quenching free radicals produced by alloxan and inhibiting tissue injuries in pancreatic β-cells.
