Effect of bortezomib on migration and invasion in cervical carcinoma HeLa cell
10.1016/j.apjtm.2015.05.004
- Author:
Chong SHI
1
;
Guo-Bin ZHANG
2
;
Shu-Wang YIN
3
Author Information
1. Department of Genetic Reproduction, Maternal, Child Health Hospital
2. Department of Graduate, Hebei United University
3. Pharmacy Department, Affiliated Hospital of North China Coal Medical University
- Publication Type:Journal Article
- Keywords:
Akt/mTOR signal pathway;
Bortezomib;
Cervical carcinoma HeLa cell;
Invasion;
Migration
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(6):485-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism. Methods: The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot. Results: MTT assay indicated bortezomib (2.5μM, 5μM, 10μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9. Conclusions: These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.
