Monascus pilosus-fermented black soybean inhibits lipid accumulation in adipocytes and in high-fat diet-induced obese mice
10.1016/S1995-7645(14)60330-8
- Author:
Young-Sil LEE
1
;
Bong-Keun CHOI
1
;
Jinhua CHENG
1
;
Seung Hwan YANG
1
;
Joo-Won SUH
1
;
Hae Jin LEE
2
;
Seung Hwan YANG
2
;
Joo-Won SUH
2
;
Dong-Ryung LEE
3
;
Won-Keun LEE
3
;
Joo-Won SUH
3
Author Information
1. Center for Nutraceutical and Pharmaceutical Materials, Myongji University
2. Interdisciplinary Program of Biomodulation, Myongji University
3. Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University
- Publication Type:Journal Article
- Keywords:
Adipocytes;
Adipogenesis-related genes;
Anti-obesity;
Black soybean;
High-fat diet-induced obese mice;
Monascus pilosus
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(4):276-282
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the anti-obesity effects and the mechanism of action of Monascus pilosus(M. pilosus)-fermented black soybean (MFBS) extracts (MFBSE) and MFBS powders (MFBSP) in adipocytes and high-fat diet (HFD)-induced obese mice, respectively. Methods: Black soybean was fermented with M. pilosus, and the main constituents in MFBS were analyzed by HPLC analysis. In vitro, MFBSE were examined for anti-adipogenic effects using Oil-Red O staining. In vivo, mice were fed a normal-fat diet (NFD) control, HFD control or HFD containing 1 g/kg MFBSP for 12 weeks, and then body weight gain and tissues weight measured. Real-time PCR and western blot assay were used to determine the mechanism of anti-adipogenic effects. Results: MFBSE inhibited lipid accumulation in 3T3-L1 adipocytes without exerting cell cytotoxicity. MFBSP treatment in HFD-fed mice significantly decreased the body weight gain compared with the HFD control mice. MFBSE and MFBSP treatment resulted in significantly lower mRNA levels of adipogenesis-related genes, such as peroxisome proliferator-activated receptor γ(PPAR γ), fatty acid-binding protein 4 (FABP4), and fatty acid synthase (FAS), in adipocytes and in white adipose tissue (WAT) of HFD-induced obese mice. Conclusions: These results suggest that the anti-obesity effects of MFBS are elicited by regulating the expression of adipogenesis-related genes in adipocytes and WAT of HFD-induced obese mice.
