Suppressive effect of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on inflammation by regulation of NF- κ B pathway and interleukin-17 in mice with dextran sulphatesodium-induced ulcerative colitis
10.1016/S1995-7645(14)60306-0
- Author:
Xin-Pu MIAO
1
;
Xiao-Ning SUN
1
;
Lu-Jia CUI
1
;
Qin-Fang CAO
1
;
Tao-Zhi DENG
1
;
Dong-Yan ZHANG
1
;
Gui-Feng ZHUANG
2
Author Information
1. Department of Gastroenterology, Hainan Provincial People's Hospital
2. Department of Gastroenterology, The Affiliated Hospital of Hainan Medical College
- Publication Type:Journal Article
- Keywords:
Dextran sulfate sodium-induced colitis;
Interleukin-17;
Nuclear factor;
Pectic polysaccharides;
Ulcerative colitis
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(2):147-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model. Methods: Experimental colitis was induced by dextran sulfate sodium (DSS), and mice were divided into 4 groups: control, DSS alone, DSS plus SASP, DSS plus pectic polysaccharides. The disease activity index (DAI) and histological score were observed. The tumor necrosis factor (TNF)- α and interleukin (IL)-17 levels were measured by enzyme-linked immunosorbent assay. I κ B and NF- κ B p65 expression were assessed by western blot analysis. Myeloperoxidase (MPO) activity was determined by using MPO assay kit. Results: Administration of pectic polysaccharides significantly reduced the severity of DSS-induced colitis as assessed by DAI and histological score, and resulted in down regulation of MPO activity and NF- κ B p65 expression and subsequent degradation of I κ B protein, strikingly reduced the production of TNF- a and IL-17. Conclusions: Pectic polysaccharides extracted from Rauvolfia verticillata (Lour.)Baill.var. hainanensis Tsiang exerts beneficial effects in experimental colitis and may therefore provide a useful therapeutic approach for the treatment of UC.
