Effect of cyclophosphamide on fungal infection in SLE mice detected by fluorescent quantitative PCR
10.1016/j.apjtm.2015.11.001
- Author:
Hong-Fen GE
1
;
Hong-Fen GE
2
;
Hong-Fen GE
3
;
Guan-Zhi CHEN
3
;
Min PAN
3
;
Hui GUO
4
Author Information
1. Shandong Provincial Hospital for Skin Diseases, Shandong University
2. Shandong Provincial Institute of Dermatology and Venereology, Shandong Academy of Medical Sciences
3. Department of Dermatology, The Affiliated Hospital of Qingdao University
4. Laboratory Department, The Affiliated Hospital of Qingdao University
- Publication Type:Journal Article
- Keywords:
Animal model;
Fungus;
Polymerase chain reaction;
Systemic lupus erythematosus
- From:
Asian Pacific Journal of Tropical Medicine
2015;8(12):1033-1037
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of the cyclophosphamide (CTX) on systemic lupus erythematosus mice by fluorescent quantitative polymerase chain reaction. Methods: (BALB/c × C57BL/J6) F1 hybrid mice were used as recipients of donor lymphocytes which were injected intravenously. Mice were randomly divided into control group and treatment group, serum autoantibody and urinary protein were measured, renal pathological changes and the situation of fungal infection were observed. Results: The reduced urinary protein, ameliorated renal lesions were found in the CTX-treated mice. Candida albicans and Aspergillus flavus were detected respectively in respiratory tract and gastrointestinal tract. Conclusions: CTX can alleviate lupus nephritis and the fluorescent quantitative PCR is a rapid and sensitive method for the detection of fungal infection.
