MEK/ERK signaling pathway in apoptosis of SW620 cell line and inhibition effect of resveratrol
10.1016/j.apjtm.2015.12.010
- Author:
Hao CHEN
1
;
Zhi-Liang JIN
1
;
Hai XU
2
Author Information
1. Jingzhou Central Hospital
2. Huangjiahu Hospital, Hubei University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Colon cancer;
Inhibition of proliferation;
MEK/ERK signaling pathway;
Resveratrol
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the involvement of MAPK MEK/ERK signaling transduction pathway in the apoptosis process of SW620 tumor cell line and the inhibition effect of resveratrol. Methods: SW620 cell lines were divided into 5 groups, namely, control group, PD98059 group, low-dose resveratrol group, mid-dose resveratrol group and high-dose resveratrol group. The inhibition rate of cell proliferation was detected by MTT method. The expression of apoptotic molecules and MEK/ERK signaling pathway related proteins were assayed by real-time PCR and Western blotting. Results: Compared with control group, the proliferation of cells treated with resveratrol was significantly inhibited. In the case of apoptotic molecules, the expression of Bax, Caspase 3 and Caspase 9 was increased significantly while the expression of anti-apoptotic molecule Bcl2 was decreased significantly in resveratrol groups with a dose-dependent manner. In the case of molecules in MEK/ERK signaling pathway, the expression of Ras, Raf, MEK and ERK1/2 was decreased significantly in resveratrol groups with a dose-dependent manner. Conclusions: PD98059 and resveratrol can effectively inhibit the proliferation of SW620 through inhibiting the MEK/ERK signaling pathway.
