Effect of lentivirus-mediated integrin αVβ3-shRNA on tumor growth of mice with lung cancer xenografts
10.1016/j.apjtm.2016.01.004
- Author:
Tao LIANG
1
;
Jian CHU
1
;
Dao-Xi WANG
1
;
Yong-Fu MA
2
;
Yang LIU
2
Author Information
1. Department of Thoracic Surgery, Second Artillery General Hospital of Chinese PLA
2. Department of Thoracic Surgery, Chinese PLA General Hospital
- Publication Type:Journal Article
- Keywords:
Angiogenesis;
Apoptosis;
Integrin αVβ3;
Invasion;
Lung cancer;
Xenografts
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(2):164-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of lentivirus-mediated integrin αVβ3-shRNA on tumor growth of mice with lung cancer xenograft. Methods: Lung cancer tissue, paracancer tissue and normal tissue were collected and integrin αVβ3 expression was detected; BALB/c nude mice were selected, divided into integrin αVβ3 knockdown group (KD group) and negative control group (NC group), and inoculated with cells stably infected by integrin αVβ3-shRNA lentivirus and cells stably infected by negative control-shRNA lentivirus, respectively, the growth of tumor tissue was continuously observed, and the number of apoptosis cells as well as the expression of angiogenesis, apoptosis and invasion genes in tumor tissue were detected. Results: mRNA content and protein content of integrin αVβ3 in lung cancer tissue were significantly higher than those in paracancer tissue and normal tissue; increasing trend of tumor tissue volume of KD group was weaker than that of NC group, and tumor volume at various points in time of KD group was lower than that of NC group; mRNA contents and protein contents of VEGF, FGF, EGF, Bcl-2, MMP-9, MMP-12 and MMP-13 in tumor tissue of KD group were lower than those of NC group, and apoptosis index as well as mRNA content and protein content of Bax were higher than those of NC group. Conclusions: The expression of integrin αVβ3 increases in lung cancer tissue, and lentivirus-mediated integrin αVβ3-shRNA can inhibit tumor growth of mice with lung cancer xenografts.
