Dengue virus non-structural 1 protein interacts with heterogeneous nuclear ribonucleoprotein H in human monocytic cells
10.1016/j.apjtm.2016.01.015
- Author:
Drishya DIWAKER
1
;
Drishya MISHRA
1
;
Drishya GANJU
1
;
Drishya SINGH
1
Author Information
1. Immunomodulation Lab, Defence Institute of Physiology and Allied Sciences
- Publication Type:Journal Article
- Keywords:
Dengue virus;
Heterogeneous nuclear ribonucleoprotein H;
Non-structural 1 protein;
Protein interactions
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(2):112-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study protein-protein interaction between heterogeneous nuclear ribonucleoprotein H (hnRNP H) and Dengue virus (DENV) proteins. Methods: DENV proteins were screened against the host hnRNP H protein, in order to identify the host-viral protein-protein interactions in DENV infected THP-1 cells by co-immunoprecipitation. The co-localization of the interacting proteins was further confirmed by immunofluorescence microscopy. Results: The host protein hnRNP H was found to interact with DENV non-structural 1 protein and help the virus to multiply in the cell. Conclusions: The non-structural 1 glycoprotein is a key modulator of host immune response and is also involved in viral replication. Therefore, disruption of this key interaction between hnRNP H and DENV non-structural 1 could be an important therapeutic strategy for management of DENV infection.
