Recognition of a multiple antigen peptide containing sequence from mimotope of the dengue type 3 virus NS4B protein by human antibodies
10.1016/j.apjtm.2016.01.019
- Author:
Nevis AMIN
1
;
Alicia AGUILAR
1
;
Rolando OCHOA
1
;
Armando ACOSTA
1
;
Maritza PUPO
2
;
Susana VÁZQUEZ
2
;
Yamira CABALLERO
2
;
María G. GUZMÁN
2
Author Information
1. Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute
2. 'Pedro Kourí' Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector
- Publication Type:Journal Article
- Keywords:
Antibodies;
Dengue virus;
MAP;
NS4B
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the recognition of NS4B mimotope, as multiple antigen peptide (MAP), by dengue antibodies presents in serum samples from patients with different serotype infections. Methods: A MAP containing mimotope sequence was synthesized and used to evaluate the recognition of NS4B mimotope as MAP by a panel of 66 human sera from dengue cases by an indirect ELISA assay. Results: The MAP differentiated between sera from dengue viruses infected patients and sera from healthy individuals and the best reactivity was shown by serum from dengue type 3 virus patients. The recognition was more intense with serum from patients with secondary infection. Conclusions: The findings suggest the potential use of NS4B mimotope on the development of a multi-epitope diagnostic tool. These results are important for further immunogenicity studies.
