Hepatoprotective effects of Nigella sativa seed extract against acetaminophen-induced oxidative stress
10.1016/j.apjtm.2016.01.039
- Author:
Gareeballa Osman ADAM
1
;
Gareeballa Osman ADAM
2
;
Md. Mahbubur RAHMAN
2
;
Gi-Beum KIM
2
;
Hyung-Sub KANG
2
;
Jin-Shang KIM
2
;
Shang-Jin KIM
2
;
Sei-Jin LEE
3
Author Information
1. Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Sudan University of Science and Technology
2. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus
3. Korea Basic Science Institute Jeonju Center
- Publication Type:Journal Article
- Keywords:
Acetaminophen;
Antioxidants;
Nigella sativa;
Oxidative stress;
Rat;
TIB-73 cells
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(3):221-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effects of Nigella sativa seed extract (NSSE) against acetaminophen (APAP)-induced hepatotoxicity in TIB-73 cells and rats. Methods: Toxicity in TIB-73 cells was induced with 10 μmol/L APAP and the protective effects of NSSE were evaluated at 25, 50, 75, 100 μg/mL. For in vivo examination, a total of 30 rats were equally divided into five experimental groups; normal control (vehicle), APAP (800 mg/kg body weight single IP injection) as a hepatotoxic control, and three APAP and NS pretreated (2 weeks) groups (APAP + NSSE 100 mg; APAP + NSSE 300 mg and APAP + NSSE 900 mg/kg). Results: TIB-73 cell viability was drastically decreased by (49.0 ± 1.9)% after the 10 μmol/LAPAP treatment, which also increased reactive oxygen species production. Co-treatment with NSSE at 25, 50, 75, and 100 μg/mL significantly improved cell viability and suppressed reactive oxygen species generation. In vivo, the APAP induced alterations in blood lactate levels, pH, anionic gap, and ion levels (HCO
