In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm
10.1016/j.apjtm.2016.03.008
- Author:
Azeana ZAHARI
1
;
Yasodha SIVASOTHY
1
;
Khalijah AWANG
1
;
Abdulwali ABLAT
2
;
Jamaludin MOHAMAD
2
;
Muhammad I. CHOUDHARY
3
Author Information
1. Department of Chemistry, Faculty of Science, University of Malaya
2. Institute of Biological Sciences, Faculty of Science, University of Malaya
3. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi
- Publication Type:Journal Article
- Keywords:
Antioxidant;
Antiplasmodial;
Bisbenzylisoquinoline;
Laurotetanine;
Norstephasubine;
Oxidative stress
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(4):328-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromethane extract of Alseodaphne corneri. Methods: Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography. The antiplasmodial activity of the isolated compounds was evaluated using the histidine-rich protein II assay. The isolated alkaloids were also tested for their antioxidant activity using three different assays; DPPH, ferric reducing ability of plasma and metal chelating assays. Results: Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoptosis. The antioxidant properties of the alkaloids under investigation revealed that in addition to the antiplasmodial activity, the alkaloids could also prevent oxidative stress. (+)-laurotetanine and (+)-norstephasubine exhibited strong antiplasmodial activities with IC
