Ethanol extract of Kalopanax septemlobus leaf inhibits HepG2 human hepatocellular carcinoma cell proliferation via inducing cell cycle arrest at G
10.1016/j.apjtm.2016.03.003
- Author:
Cheol PARK
1
;
Ji-Suk JEONG
2
;
Ji-Suk JEONG
3
;
Yong-Joo KIM
3
;
Jin-Woo JEONG
4
;
Yung Hyun CHOI
4
;
Sung Ok KIM
5
;
Gi-Young KIM
6
;
Su-Hyun HONG
7
;
Yung Hyun CHOI
7
Author Information
1. Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dongeui University
2. Department of Research and Development of Resources, Gurye-gun Agricultural Center
3. Wild Flower Institute
4. Anti-Aging Research Center, Dongeui University
5. Department of Food Science and Biotechnology, College of Engineering, Kyungsung University
6. Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University
7. Department of Biochemistry, Dongeui University College of Korean Medicine
- Publication Type:Journal Article
- Keywords:
CDK inhibitor;
G;
Hepatocellular carcinoma;
Kalopanax septemlobus;
PRB
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(4):344-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of an ethanol extract of Kalopanax septemlobus (Thunb.) Koidz. leaf (EEKS) on cell proliferation in human hepatocellular carcinoma cells and its mechanisms of action. Methods: Cells were treated with EEKS and subsequently analyzed for cell proliferation and flow cytometry analysis. Expressions of cell cycle regulators were determined by reverse transcriptase polymerase chain reaction analysis and Western blotting, and activation of cyclin-associated kinases studied using kinase assays. Results: The EEKS suppressed cell proliferation in both HepG2 and Hep3B cells, but showed a more sensitive anti-proliferative activity in HepG2 cells. Flow cytometry analysis revealed an association between the growth inhibitory effect of EEKS and with G
